Despite Aniston divorcing ex-husband Brad Pitt over 12 years ago, Johnson was clearly keen to see how Aniston and Jolie – who married Pitt after his split from the Friends actress – would cope with being in the same room.
Aniston joined Carol Brunett to present an award at the Beverly Hilton Hotel – and eagle-eyed fans captured the moment Johnson tried to subtly catch a glimpse of Jolie’s reaction.
Johnson was pictured taking a side-eyed glance at the Tomb Raider actress who averted her eyes from the stage.
But Jolie was forced to stand as the audience applauded Brunett with a standing ovation.
The picture quickly went viral with one user tweeting: “You’re lying if you can’t relate to Dakota staring at Angelina’s face for a reaction to Jennifer being on stage.”
Another posted: “Lol at Angelina Jolie participating in a standing ovation that’s half for Jennifer Aniston.”
US actor Armie Hammer’s wife Elizabeth Chambers was less subtle and appeared to look directly at Jolie.
Aniston and Jolie’s relationship was far from amicable for years after Pitt left Aniston for the Maleficent actress when they met on the set of Mr and Mrs Smith in 2005. They went on to marry in 2014 before Jolie filed for divorce in 2016.
Pitt formally adopted Jolie’s two adopted children Maddox, 16, and Zahara, 13, after they had been together for two years.
The couple later adopted Pax, 14, and then had three biological children together – Shiloh, 11 and nine-year-old twins Knox and Vivienne.
Laura Dern, Nicole Kidman, Zoe Kravitz, Reese Witherspoon and Shailene Woodley pose in the press room with the award for best television limited series or motion picture made for television for “Big Little Lies” at the 75th annual Golden Globe Awards at the Beverly Hilton Hote
Kendall Jenner arrives for the 75th Golden Globe Awards
Heidi Klum kisses Emily Ratajkowski at a 2018 Golden Globe Awards After Party
Tom Hanks goes to the bar to get his own drinks at t the 75th Annual Golden Globe Awards
Oprah Winfrey speaks after accepting the Cecil B. Demille Award at the 75th Golden Globe Awards in Beverly Hills
Seth Meyers hosts the 75th Golden Globe Awards in Beverly Hills
Paul Drinkwater/Courtesy of NBC via Reuters
Willem Dafoe reacts to host Seth Meyers at the 2017 Golden Globe Awards
Presenter Natalie Portman and Ron Howard at the 75th Golden Globe Awards in Beverly Hills
Paul Drinkwater/Courtesy of NBC via Reuters
Ewan McGregor accepting the award for best actor in a limited series or motion picture made for TV for his role in “Fargo,” at the 75th Annual Golden Globe Awards in Beverly Hills
Paul Drinkwater/NBC via AP
James Franco accepting the award for best actor in a motion picture comedy or musical for his role in “The Disaster Artist,” as Tommy Wiseau, left, and brother Dave Franco look on at the 75th Annual Golden Globe Awards in Beverly Hills
The cast and crew of “Three Billboards Outside Ebbing, Missouri”
Angelina Jolie attends The 75th Annual Golden Globe Awards
Ewan McGregor wears a “Time’s Up” pin on his all-black tuxedo
Laura Dern accepting the award for best supporting actress in a series, limited series or motion picture made for TV for her role in “Big Little Lies,” at the 75th Annual Golden Globe Awards
Naomi Campbell arrives at the 75th annual Golden Globe Award
Chris Hemsworth and Jessica Chastain
Sterling K. Brown accepting the award for best actor in a TV drama series for his role in “This Is Us,” at the 75th Annual Golden Globe Awards in Beverly Hills
Gal Gadot arrives at the 75th annual Golden Globe Awards
Helen Mirren and Viola Davis
Millie Bobby Brown
Margot Robbie arrives at the 75th annual Golden Globe Awards
Aziz Ansari accepting the award for best actor in a comedy series for his role in “Master of None,” at the 75th Annual Golden Globe Awards in Beverly Hills
Heidi Klum arrives at the 75th annual Golden Globe Awards
Emma Stone and Shirley MacLaine
Emilia Clarke and Kit Harington
Frances McDormand wins Best Performance by an Actress in a Motion Picture Drama for “Three Billboards Outside Ebbing, Missouri” at the 75th Golden Globe Awards
Presenters Geena Davis and Susan Sarandon
Claire Foy and Matt Smith arrive
Kirk Douglas and Catherine Zeta Jones
Roseanne Barr and John Goodman, presenters at the 75th Golden Globe Awards in Beverly Hills
Presenters Emma Watson and Robert Pattinson
Sharon Stone and son Roan Joseph Bronstein
Justin Timberlake and Jessica Biel